Identification of L

Add time:08/04/2019    Source:sciencedirect.com

Butyrivibrio fibrisolvens strain X6C61 produces two extracellular polysaccharides (EPS-I and EPS- II) separable by anion-exchange chromatography. The neutral sugar constituents of EPS-I were identified by gas-liquid chromatography (GLC) as the alditol acetates of rhamnose, mannose, galactose, glucose, and an unidentified component. These results were confirmed using thin-layer chromatography (TLC). Neutral sugar analysis of EPS-II, which eluted from DEAE-Sephadex at 0.4 M NaCl, yielded the alditol acetates of rhamnose, galactose, glucose, and idose. However, idose was not found when hydrolysates of EPS-II were analysed by TLC. Further investigations showed that the iditol hexaacetate detected via GLC was an artifact of the commonly-used procedures for neutral sugar analysis. This compound was instead generated from L-iduronic acid, as shown by GLC-MS studies.

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