Determination of 3′-azido-3′-deoxythymidine, 2′,3′-dideoxycytidine, 3′-fluoro-3′-deoxythymidine and 2′,3′-dideoxyinosine in biological samples by high-performance liquid chromatography

Add time:08/05/2019    Source:sciencedirect.com

A simple and fast high-performance liquid chromatographic assay for the determination of 3′-azido-3′-deoxythymidine (AZT), 2′,3′-dideoxycytidine (ddC), 3′-fluoro-3′-deoxythymidine (FT) and 2′,3′-dideoxyinosine (ddI) in complex biological matrices is described. The method allows rapid nucleoside determination using a phenyl column within extra- as well as intracellular media without further sample pretreatment and extraction procedures. The lower limit of detection is ca. 0.05 μg/ml for each nucleoside, and the separation can easily be optimized for AZT, ddC, FT and ddI by variation of the methanolic part of the mobile phase and the detector wavelengths.

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