The polymerization of adenosine 5′-diphosphate-ribose moiety of NAD by nuclear enzyme

Add time:08/07/2019    Source:sciencedirect.com

The radioactive acid-insoluble material, Product I, which was formed by rat-liver nuclear preparation from labeled NAD or labeled ATP with nicotinamide mononucleotide, has been partially purified. It has a peak at 3 S with sucrose density-gradient centrifugation, and a density of around 1.57 with Cs2SO4 equilibrium density-gradient centrifugation.Product I was converted into the acid-soluble form by snake-venom phosphodiesterase. Product I was not digested by micrococcal nuclease, spleen phosphodiesterase, potato phosphodiesterase, potato nucleotide pyrophosphatase, spleen NAD nucleosidase, or trypsin.The radioactive acid-soluble substance, Product II, which was released by venom-phosphodiesterase digestion, was isolated from a Dowex 1 formate column. Product II showed absorption maxima at 261, 259, and 258 mμ under alkaline neutral and acidic conditions, respectively. The molar ratio of adenine (as adenosine), ribose, phosphorus and periodate consumption was 1:2:2:1. All phosphates were converted to inorganic phosphate by acid-phosphatase digestion.Structures of Products I and II were proposed.

We also recommend Trading Suppliers and Manufacturers of ADENOSINE-8-14C 5'-DIPHOSPHATE AMMONIUM (cas 134907-90-7). Pls Click Website Link as below: cas 134907-90-7 suppliers

Prev:Effects of suramin on increases in cytosolic calcium and on inhibition of adenylate cyclase induced by adenosine 5′-diphosphate in human platelets
Next:[18] Synthesis of ribulose 1,5-bisphosphate: Routes from glucose 6-phosphate (via 6-phosphogluconate) and from adenosine monophosphate (via ribose 5-phosphate))