Preparation of D-valine from D,L-5-Isopropylhydantoin (cas 16935-34-5) by stereoselective biocatalysis

Add time:08/04/2019    Source:sciencedirect.com

The synthesis of D-valine has been optimized starting from isobutyraldehyde, sodium cyanide and ammonium carbonate. The intermediate hydantoin was converted by a bienzymatic system to D-valine.The specific consumption of Agrobacterium radiobacter resting cells, which are high producers of hydantoinase and N-carbamoyl-D-amino acid amidohydrolase, has been evaluated and the optimum reaction conditions were obtained by using a 10% solution of the hydantoin and a hydantoin/cells w/w ratio equal to 5, at 40 °C for 48 h.Further, several methods have been optimized to recover the amino acid from the hydrolysate.The final yield was about 60% referred to the starting aldehyde.

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