Simultaneous Determination of Chloroprocaine Hydrochloride and Its Degradation Product 4-Amino-2-chlorobenzoic Acid in Bulk Drug and Injection Solutions by High-Performance Liquid Chromatography

Add time:07/14/2019    Source:sciencedirect.com

A high-performance liquid chromatographic method has been developed for the simultaneous determination of chloroprocaine hydrochloride and its hydrolytic degradation product, 4-amino-2-chlorobenzoic acid. Separation is achieved using a μ-Bondapak C18 column and the eluant, water-acetonitrile-methanol-glacial acetic acid (74:20:5:1) containing 0.05-0.08% (w/v) sodium 1-heptanesulfonate, at a flow rate of 2 mL/min. Benzoic acid and p-nitroacetophenone were used as internal standards. A variable-wavelength UV detector (278 nm) was used for detection of the compounds. The method is simple, accurate, and precise.

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