Preparation of specific antisera to estradiol 17-glucuronide

Add time:08/06/2019    Source:sciencedirect.com

The preparation and antigenic properties of three different types of estradiol 17-glucuronide-bovine serum albumin (BSA) conjugates in which the haptens are linked to the carrier protein through the C-2 (or C-4) position have been described. All antibodies raised against these antigens in rabbits possessed high affinity (KA = 2.22 × 109–2.58 × 109M−1) and excellent specificity to estradiol 17-glu-curonide, exhibiting little cross-reactivities for other estrogen glucuronides and sulphates (<1%) and no cross-reactions for free estrogens and other steroids (< 0.001%) with an only exception of testosterone 17-glucuronide (0.86–11.5%).

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