Fluorescence enhancement of yttrium(III)–rutin by nucleic acids in the presence of cetyltrimethylammonium bromide

Add time:08/10/2019    Source:sciencedirect.com

It is found that nucleic acids can enhance the fluorescence intensity of yttrium(III) (Y3+)–rutin in presence of cetyltrimethylammonium bromide (CTMAB) system. In hexamethylenetetramine (HMTA)–HCl buffer, the maximum enhanced fluorescence is produced, with maximum excitation and emission wavelengths at 452 and 520 nm, respectively. Based on this, a new fluorimetric method of determination of nucleic acids is proposed. Under optimum conditions, the enhanced fluorescence intensity is proportion to the concentration of nucleic acids in the range of 1.0 × 10−7 to 1.0 × 10−5 g/ml for fish sperm DNA (fsDNA), 1.0 × 10−7 to 4.6 × 10−6 g/ml for yeast RNA (yRNA), their detection limits (S/N = 3) are 7.5 × 10−8, 8.0 × 10−8 g/ml, respectively. The interaction mechanism is also studied.

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