Potential Anti-infective Targets in Pathogenic Yeasts: Structure and Properties of 3,4-Dihydroxy-2-butanone 4-phosphate Synthase of Candida albicans

Add time:07/12/2019    Source:sciencedirect.com

A synthetic gene specifying a putative 3,4-dihydroxy-2-butanone 4-phosphate synthase of Candida albicans directed the synthesis of a 22.5 kDa peptide in a recombinant Escherichia coli strain. The recombinant protein was purified to apparent homogeneity by two chromatographic steps and was shown to catalyze the formation of l-3,4-dihydroxy-2-butanone 4-phosphate from ribulose 5-phosphate at a rate of 332 nmol mg−1 min−1. Hydrodynamic studies indicated a native molecular mass of 41 kDa in line with a homodimer structure. The protein was crystallized in its apoform. Soaking yielded crystals in complex with the substrate ribulose 5-phosphate. The structures were solved at resolutions of 1.6 and 1.7 Å, respectively, using 3,4-dihydroxy-2-butanone 4-phosphate synthase of E. coli for molecular replacement. Structural comparison with the orthologs of Magnaporthe grisea and Methanococcus jannaschii revealed a hitherto unknown conformation of the essential acidic active-site loop.

We also recommend Trading Suppliers and Manufacturers of 2-Butanone, 1-amino-4-phenyl- (cas 46119-97-5). Pls Click Website Link as below: cas 46119-97-5 suppliers

Prev:Enantioselective reduction of 4-phenyl-2-butanone to chiral 4-phenyl-2-butanol in glycerol modified Saccharomyces cerevisiae cell culture
Next:Induction of let-7e gene expression attenuates oncogenic phenotype in HCT-116 colorectal cancer cells through targeting of DCLK1 regulation)