Analysis of phenols in water by high-performance liquid chromatography using COUMARIN-6-SULFONYL CHLORIDE (cas 10543-42-7) as a fluorogenic precolumn label

Add time:08/06/2019    Source:sciencedirect.com

A simple, sensitive and rapid reversed-phase high-performance liquid chromatography (RP-HPLC) method is proposed for the analysis of some environmentally important phenols in water. The use of coumarin-6-sulphonyl chloride (C6SCl) as a fluorescence-labeling reagent has been investigated. The compound reacts with phenols within 20 min under mild conditions (ambient temperature, pH 9.0) to give sulphonates that can be separated by RP-HPLC employing fluorescence detection at λex = 360 and λem = 460 nm. The optimum conditions for fluorescence, derivatization and chromatographic separation have been established and detection limits in the range 0.1–0.9 μg l−1 were obtained for the studied compounds. The calibration curves were linear for the range 6–200 μg l−1 for phenol, 3–200 μg l−1 for 2-chlorophenol, 4-chlorophenol and 2,3,5-trichlorophenol and for the range of 3–100 μg l−1 for 2,3-dichlorophenol and 3,5-dichlorophenol. The practical applicability of the method to environmental samples was demonstrated by analyzing drinking and industrial water samples spiked with the phenolic compounds.

We also recommend Trading Suppliers and Manufacturers of COUMARIN-6-SULFONYL CHLORIDE (cas 10543-42-7). Pls Click Website Link as below: cas 10543-42-7 suppliers

Prev:Whisker 11. Poly(ester—amide)s derived from vanillic acid and 4-aminobenzoic acid
Next:Synthesis and spectroscopic study of 2,7-diethylamino-2-oxo-2H-chromen-3-yl benzothiazole-6-sulfonyl chlorides and its derivatives)