2-Deoxy-2-fluoro-L-fucose (cas 70763-62-1) and its effect on L-[1-14C] fucose utilization in mammalian cells

Add time:07/11/2019    Source:sciencedirect.com

Summary2-Deoxy-2-fluoro-L-fucose (cas 70763-62-1) has been obtained as a chromatographically pure compound from 3,4-O-acetyl-L-fucal and its structure established by NMR measurements. Mouse fibroblasts (TAL/N P and TAL/N B) in culture incorporate L-[1-14C-] fucose into glycoproteins of trypsinates and of cell components. The labelling is competitively and progressively diminished when 2-deoxy-2-fluoro-L-fucose or L-fucose is added to the medium in the range 0.1mM to 10mM. Evidence suggests that the fluorosugar is incorporated into glycoprotein fractions.

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