Purification and characterization of three isoforms of S-adenosyl-l

Add time:08/06/2019    Source:sciencedirect.com

Three distinctly different isoforms. of S-adenosyl-l-methionine-(R,S)-tetrahydrobenzylisoquinoline-N-methyltransferases could be isolated from cell suspension cultures of Berberis koetineana. These isoforms were designated NMT-I, -II and -III. The three enzymes have different molecular weights (60–78 × 103), pH optima (6.8/7.4), kinetic properties and substrate specificities. NMT-I showed maximal activity with (R)-tetrahydropapaverine as substrate, NMT-II and -III were most active against (R)-coclaurine. The mixture of all three NMT's was immobilized on CH-Sepharose or CPG-10 glass beads. The enzymes under these conditions retained their properties and represent a useful tool for the preparative synthesis of isotopically labelled N-methylated benzylisoquinoline alkaloids.

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