High-Performance Liquid Chromatographic Determination of Isoniazid and 1 -Isonicotinyl-2-lactosylhydrazine in Isoniazid Tablet Formulations

Add time:07/12/2019    Source:sciencedirect.com

A high-performance liquid chromatographic procedure is presented for the simultaneous determination of isoniazid and 1-isonicotinyl-2-lactosylhydrazine (I) in isoniazid tablet formulations. An aliquot of a diluted aqueous tablet extract is introduced onto a microparticulate cyanopropyl bonded-phase column using a valve-loop injector and chromatographed using a mobile phase of acetonitrile-0.01 M, pH 3.5 aqueous acetate buffer (5:95). Compound I can be determined at levels as low as 0.5% of the isoniazid label claim. The relative standard deviations are 0.4 and 0.7% for the simultaneous determination of isoniazid and I, respectively. Seven commercial tablet formulations contained 93.8-97.0% of the labeled isoniazid amounts and 0.3-5.8% of I, expressed as equivalent isoniazid relative to the labeled isoniazid level.

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