Liquid scintillation counting of C14-labeled amino acids on paper, using trinitrobenzene-1-sulfonic acid, and a modified combustion apparatus

Add time:08/09/2019    Source:sciencedirect.com

The sodium salt of trinitrobenzene-1-sulfonic acid has been used to detect C14-labeled amino acid spots on paper without removing radio-activity from the carboxyl group. Combustion of these paper spots was carried out in improved combustion vessels with recoveries of radioactivity from 96 to 100%. These techniques have been used to compare the effect of chromatography and electrophoresis upon the recovery of radioactivity in labeled amino acids.

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