An enzymatic alternative for the synthesis of nucleoside 5′-monophosphates

Add time:07/13/2019    Source:sciencedirect.com

A new procedure was carried out for the synthesis of nucleoside 5′-monophosphates, involving the use of two enzymes. The first step applied phospholipase D from Streptomyces netropsis and phosphatidylcholine as phosphatidyl donor, to give 5′-(3-sn-phosphatidyl) nucleosides (C, U, A, I). These were selectively hydrolysed in the second step by the action of phospholipase C from Bacillus cereus to produce the respective 5′-nucleotides. Application of this methodology on a preparative scale conducted to 5′-adenosine monophosphate in 63% overall yield from adenosine. The regioselectivity of these enzymes avoids protection steps, the overall synthesis is performed under mild reaction conditions and product isolation is easily achieved.

We also recommend Trading Suppliers and Manufacturers of Cytidine 5'-monophosphate disodium salt (cas 6757-06-8). Pls Click Website Link as below: cas 6757-06-8 suppliers

Prev:Solubility of disodium cytidine 5′-monophosphate in different binary mixtures from 288.15 K to 313.15 K
Next:Charge transfer complexes of adenosine-5′-monophosphate and cytidine-5′-monophosphate with water-soluble cobalt(II) Schiff base complexes in aqueous solution)