Label-free signal-on aptasensor for sensitive electrochemical detection of arsenite

Add time:07/12/2019    Source:sciencedirect.com

A signal-on aptasensor was fabricated for highly sensitive and selective electrochemical detection of arsenite with a label-free Ars-3 aptamer self-assembled on a screen-printed carbon electrode (SPCE) via Au–S bond. The Ars-3 aptamer could adsorb cationic polydiallyldimethylammonium (PDDA) via electrostatic interaction to repel other cationic species. In the presence of arsenite, the change of Ars-3 conformation due to the formation of Ars-3/arsenite complex led to less adsorption of PDDA, and the complex could adsorb more positively charged [Ru(NH3)6]3+ as an electrochemically active indicator on the aptasensor surface, which produced a sensitive “turn-on” response. The target-induced structure switching could be used for sensitive detection of arsenite with a linear range from 0.2 nM to 100 nM and a detection limit down to 0.15 nM. Benefiting from Ars-3 aptamer, the proposed system exhibited excellent specificity against other heavy metal ions. The SPCE-based aptasensor exhibited the advantages of low cost and simple fabrication, providing potential application of arsenite detection in environment.

We also recommend Trading Suppliers and Manufacturers of trisodium arsenite (cas 13464-37-4). Pls Click Website Link as below: cas 13464-37-4 suppliers

Prev:Syntheses, crystal structures, antioxidant SOD-like properties and in-vitro antimicrobial studies of Cu(II) and Ni (II) complexes with 2-((E)-(4-bromo-2-chlorophenylimino) methyl)-6-bromo-4-nitrophenol and (E)-1-(3, 5-dichloro-2-hydroxybenzylidene)-4, 4-dimethylthiosemicarbazide
Next:Ligand effects on arsenite removal by zero-valent iron/O2: Dissolution, corrosion, oxidation and coprecipitation)