Reconstitution of Helix pomatia hemocyanin with cyanocuprate(I)
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Add time:08/09/2019 Source:sciencedirect.com
The possibility to reconstitute hemocyanin with copper(II) ions can be explained by the reduction of these ions by residual cyanide and by the formation of a stable copper(I) complex that introduces copper(I) into the active site of hemocyanin. The reconstitution of hemocyanin with cyanocuprate(I) in air is disturbed by a secondary reaction whereby the copper-oxygen band is destroyed irreversibly as a function of time. The formation of hydroxyl radicals at the active site of hemocyanin seems partially responsible for its destruction
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