5-Thio-2-nitrobenzoate as a circular dichroism marker to detect the tense structure of bovine hemoglobin

Add time:08/11/2019    Source:sciencedirect.com

A characteristic, strong CD absorption appeared at 312 nm, when a 5-thio-2-nitrobenzoate (TNB) group was anchored at Cys93-S(βF9) of bovine hemoglobin (Hb) through-SS-linkage. The Hill coefficient, n = 2.7 of the TNB-modified Hb was practically identical with that of native Hb, demonstrating that the marker-anchoring was made successfully, without destroying the cooperativity function. The new 312-nm CD absorption disappears concurrently with the conversion from the tense to the relaxed quaternary structure of Hb, clearly indicating that TNB is an excellent CD marker to detect the tense structure of deoxy-Hb.

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