Tetrahymena 14-nm filament-forming protein (cas 135343-59-8) has citrate synthase activity

Add time:08/10/2019    Source:sciencedirect.com

The Tetrahymena 14-nm filament-forming protein (cas 135343-59-8) (49K protein) is a structural protein involved in oral morphogenesis and in pronuclear behavior during conjugation. Cloning the 49K protein gene from a Tetrahymena thermophila cDNA library, we found that its primary structure exhibits a high sequence identity (51.5%) with porcine heart citrate synthase and retains functional domains. The 49K protein actually possesses citrate synthase activity, and is detected in mitochondria. These results suggest that the 49K protein has dual functions as both a respiratory enzyme and a structural protein in the cytoskeleton.

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