Electrical communication between a water-soluble 1,1′-dimethylferrocene-2-hydroxypropyl-β-cyclodextrin complex and glucose oxidase: biosensor applications

Add time:08/15/2019    Source:sciencedirect.com

2-Hydroxypropyl-β-cyclodextrin (HPBC) was used to enclose 1,1′-dimethylferrocene (DMF) into its hydrophobic cavity to form a yellow water-soluble complex with a molar ratio of about 6 to 1. The cyclic voltammogram of the complex solution exhibited one single electron transfer process with a half-wave potential of 170 mV. The oxidation potential of the DMF-HPBC complex was not affected by pH ranging from pH 4·7 to 8·8 and the difference between the anodic and cathodic peak potentials was observed to be 95 mV.The DMF-HPBC complex was used together with immobilized glucose oxidase to construct a mediated biosensor for the determination of β-D-glucose. The enzyme was either convalently immobilized on a preactivated Immunodyne nylon membrane or retained onto the sensing area of the electrode by a dialysis membrane. In the latter preparation, the enzyme electrode exhibited a detection limit of 0·02 mM and a linear range of 0·02 to 0·5 mM. About 2 min was required to attain 90% of full response. The biosensor was effective for the determination of glucose in food and beverage samples.

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