dihydroxyacetone sulfate (cas 13552-82-4) as substrate analogue for α-glycerol phosphate dehydrogenase

Add time:08/11/2019    Source:sciencedirect.com

dihydroxyacetone sulfate (cas 13552-82-4) is a substrate for α-glycerolphosphate dehydrogenase (sn-glycerol-3-phosphate:NAD + 2-oxidoreductase, EC 1.1.1.8). The substitution of the sulfate for the phosphate does not affect the Km of the enzyme for NADH. It affects, on the contrary, the Km of the sugar ester, which is 45- to 100-fold larger, and the pH optimum, which is shifted from 8.0 to 6.0–6.5, with dihydroxyacetone sulfate as substrate. The similarity of the plot of the pKm versus pH for dihydroxy-acetone phosphate and dihydroxyacetone sulfate suggests that the same groups are involved in the binding of the two compounds to the enzyme. The kinetic data favor the hypothesis that the dianion of dihydroxyacetone phosphate is the active form of the substrate.

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