Selective and single step adsorption of α-lactalbumin from whole cow's milk on hydroxyapatite microbeads

Add time:08/11/2019    Source:sciencedirect.com

In this study, newly modified hydroxyapatite microbeads were used to isolate α-lactalbumin (α-LA) from cow's milk by a single step. Inorganic material, pure hydroxyapatite was embedded in ethylene glycol dimethacrylate (EGDMA) in the presence of pore maker. Newly synthesized hydroxyapatite microbeads were used firstly for adsorption of α-LA from aqueous solution. The maximum adsorption capacity of α-LA from aqueous solution was observed at pH 7.0 (10.0 mM phosphate buffer) at 25 °C in a batch system, in which parallel separate experiments were performed independently. Synthesized hydroxyapatite microbeads and pure hydroxyapatite were used for adsorption studies from whole milk (without pre-treatment such as fat and caseins removal). Our method is cheap, simple, efficient and rapid. The results showed that modified hydroxyapatite microbeads had selective affinity to α-LA because of good adsorption properties.

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