On the inhibition of deoxycytidylate hydroxymethylase by 5-fluoro-2′-deoxycytidine 5′-monophosphate

Add time:07/12/2019    Source:sciencedirect.com

Studies were performed to determine whether 5-fluoro-2′-deoxycytidine 5′-monophosphate (FdCMP) is an inhibitor of deoxycytidylate hydroxymethylase and whether it could form an isolable covalent complex with the enzyme and the cofactor, 5,10-methylenetetrahydrofolate. The results showed that although FdCMP is a competitive inhibitor of dCMP hydroxymethylase, it does not cause time-dependent inhibition of the enzyme in the presence of cofactor. Further, although uv difference spectral evidence was found for a FdCMP-cofactor-enzyme complex, the complex was not sufficiently stable to isolate on nitrocellulose filters. We conclude that FdCMP is not a mechanism-based inhibitor of dCMP hydroxymethylase.

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