Research reportTheoretical and experimental epitope mapping of thymosin β4

Add time:08/12/2019    Source:sciencedirect.com

Two rabbit polyclonal antisera, one directed against thymosin β4 and the other one against the peptide fragment thymosin β4 (1–14) were characterised by epitope mapping. Hexapeptides representing the whole sequence of the native peptide and overlapping by one amino acid were synthesised on polystyrene pins. The antigenic determinants were identified in microtitre plates with an ELISA procedure. The polyclonal serum against thymosin β4 detected three epitopes (N-terminal, mid-region and C-terminal) whereas the polyclonal serum against the fragment contained only antibodies specific for the N-terminal epitope. These experimental results are consistent with theoretical predictions.

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