Determination of α-amanitin and β-amanitin in human biological fluids by high-performance liquid chromatography

Add time:08/18/2019    Source:sciencedirect.com

A high-performance liquid chromatographic assay of α-amanitin and β-amanitin in human serum, urine, or stomach washings is described. Sample preparation involves a chemical step with deproteinization and organic solvent treatment, and a selective cleanup and concentration step on reversed-phase prepacked cartridges. Separations are performed on a reversed-phase analytical column under isocratic conditions with uv detection at 280 nm. The method allows the quantitation of α- and β-amanitin separately with a detection limit of 10 ng/ml for both toxins.

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