MicroRNA regulation of DNA repair gene expression in 4-aminobiphenyl-treated HepG2 cells
-
Add time:08/13/2019 Source:sciencedirect.com
We examined the role of miRNAs in DNA damage response in HepG2 cells following exposure to 4-aminobiphenyl (4-ABP). The arylamine 4-ABP is a human carcinogen. Using the Comet assay, we showed that 4-ABP (18.75–300 μM) induces DNA damage in HepG2 cells after 24 h. DNA damage signaling pathway-based PCR arrays were used to investigate expression changes in genes involved in DNA damage response. Results showed down-regulation of 16 DNA repair-related genes in 4-ABP-treated cells. Among them, the expression of selected six genes (UNG, LIG1, EXO1, XRCC2, PCNA, and FANCG) from different DNA repair pathways was decreased with quantitative real-time PCR (qRT-PCR). In parallel, using the miRNA array, we reported that the expression of 27 miRNAs in 4-ABP-treated cells was at least 3-fold higher than that in the control group. Of these differential 27 miRNAs, the most significant expression of miRNA-513a-5p and miRNA-630 was further validated by qRT-PCR, and was predicted to be implicated in the deregulation of FANCG and RAD18 genes, respectively, via bioinformatic analysis. Both FANCG and RAD18 proteins were found to be down-regulated in 4-ABP-treated cells. In addition, overexpression and knockdown of miRNA-513a-5p and miRNA-630 reduced and increased the expression of FANCG and RAD18 proteins, respectively.Based on the above results, we indicated that miRNA-513a-5p and miRNA-630 could play a role in the suppression of DNA repair genes, and eventually lead to DNA damage.
We also recommend Trading Suppliers and Manufacturers of 4-Aminobiphenyl -D-Glucuronide Sodium Salt (cas 116490-30-3). Pls Click Website Link as below: cas 116490-30-3 suppliers
Prev:Design and synthesis of novel imidazo[4,5-b]pyridine based compounds as potent anticancer agents with CDK9 inhibitory activity
Next:ArticlePoly(4-aminobiphenyl): chemical synthesis, characterization studies and comparison with conductive electropolymerized samples) - 【Back】【Close 】【Print】【Add to favorite 】
- Related Information
- Microextraction by packed sorbent and salting-out-assisted liquid–liquid extraction for the determination of aromatic amines formed from azo dyes in textiles08/21/2019
- Research letterDevelopment of sarcomas in heterotopically transplanted rat urinary bladder unit exposed to glucuronic acid conjugate of N-hydroxy-4-aminobiphenyl☆08/20/2019
- Comparison of the mutagenicities of 4-aminobiphenyl and benzidine in the Salmonella/microsome, Salmonella/hepatocyte and host-mediated assays08/19/2019
- New syntheses of N-(guanosin-8-yl)-4-aminobiphenyl and its 5′-monophosphate08/18/2019
- Synthesis of the urinary glucuronic acid conjugate of n-hydroxy-4-aminobiphenyl*08/17/2019
- The metabolic activation of 2-aminofluorine, 4-aminobiphenyl, and benzidine by cytochrome P-450-107S1 of Pseudomonas aeruginosa08/16/2019
- Substituent effects on the in vitro and in vivo genotoxicity of 4-aminobiphenyl and 4-aminostilbene derivatives08/15/2019
- ArticlePoly(4-aminobiphenyl): chemical synthesis, characterization studies and comparison with conductive electropolymerized samples08/14/2019
