Separation of saturated, mono-unsaturated and di-unsaturated aldehydes as 2,4-dinitrophenylhydrazones using high-performance liquid chromatography at increased temperature☆

Add time:08/14/2019    Source:sciencedirect.com

Saturated and mono- and di-unsaturated aldehydes (C5C10) were converted into their 2,4-dinitrophenylhydrazones. The separation of nanogram amounts of these hydrazones was performed by high-performance liquid chromatography with C18 reversed-phase columns in an isocratic elution. Separation of all aldehydes tested could noly be achieved at an elevated temperature (50°C). A linear relationshi between log k′ and the carbon number for a series of homologous compounds could be observed also at an elevated temperature. An optimal separation was achieved utilizing the methylene group selectivity which was found to be temperature dependent.Applicatin of this method shows the advantage of an optimized separation for the determination of carbonyl compounds from the oxidation of fatty acids in meat.

We also recommend Trading Suppliers and Manufacturers of TRANS-2-NONENAL 2,4-DINITROPHENYLHYDRAZONE (cas 18287-00-8). Pls Click Website Link as below: cas 18287-00-8 suppliers

Prev:The formation of cis-3-nonenal, trans-2-nonenal and hexanal from linoleic acid hydroperoxide isomers by a hydroperoxide cleavage enzyme system in cucumber (Cucumis sativus) fruits
Next:Determination of the lipid peroxidation product trans-4-hydroxy-2-nonenal in biological samples by high-performance liquid chromatography and combined capillary column gas chromatography—negative-ion chemical ionisation mass spectrometry)