An efficient deprotection method for 5′-[O,O-bis(pivaloyloxymethyl)]-(E)-vinylphosphonate containing oligonucleotides

Add time:08/13/2019    Source:sciencedirect.com

5′-(E)-Vinylphosphonate (VP) is an effective bioisostere of the natural 5′-monophosphate in small interfering RNAs (siRNAs). Solid-phase synthesis of VP-siRNAs requires the use of appropriately protected VP-phosphoramidites in combination with optimal oligonucleotide deprotection conditions. Addition of 3% (v) neat diethylamine to the standard aqueous ammonia deprotection conditions allows clean and rapid one-step deprotection of 5′-[O,O-bis(pivaloyloxymethyl)] (POM)-protected VP oligonucleotides, minimizing side reactions and impurities, which broadly enhances the scope of VP oligonucleotide synthesis.

We also recommend Trading Suppliers and Manufacturers of bis(Trimethylsilyl) Vinylphosphonate (cas 18291-41-3). Pls Click Website Link as below: cas 18291-41-3 suppliers

Prev:Review articleEffects of l-norgestrel on the endothelium-dependent relaxation response of rabbit clitoral cavernous smooth muscles
Next:Synthesis of 2-(arylamino)ethyl phosphonic acids via the aza-Michael addition on diethyl vinylphosphonate)