PaperEnzymatic continuous production of N-acetyl-l-methionine from N-acetyl-dl-methioninamide with Erwinia carotovora containing a new amidase activity

Add time:08/14/2019    Source:sciencedirect.com

A procedure for production of N-acetyl-l-methionine (N-Ac-l-Met) from its racemic mixture by enzymatic continuous hydrolysis of the l-enantiomer of N-acetyl-dl-methioninamide (N-Ac-dl-Met · NH2) is described. For this hydrolysis, whole cells of Erwinia carotovora immobilized by κ-carrageenan gel were employed. The complete conversion of N-Ac-dl-Met · NH2 to N-Ac-l-Met was achieved by using a column packed with the immobilized cells. The half-life of the enzyme activity of the column was calculated to be ∼100 days. Pure crystalline N-Ac-l-Met was readily isolated from the effluent with >87.5% yield. N-Ac-d-Met · NH2 was easily racemized by incubating in ethanol containing 0.1 m NaOH for 30 min at 80°C, and used again for substrate.

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