Karyotype analysis of Piptanthus concolor based on FISH with a oligonucleotides for rDNA 5S

Add time:07/13/2019    Source:sciencedirect.com

The karyotype of Piptanthus concolor was analysed via fluorescence in situ hybridization (FISH) using oligonucleotide probe for ribosomal DNA (rDNA) (5S rDNA, 41 bp). P. concolor had 2n = 18 chromosomes. The karyotype was composed of 3 pairs of metacentric chromosomes (including one pair of satellites), 3 pairs of submetacentric chromosomes, and 3 pairs of subtelocentric chromosomes. 5S rDNA loci were detected in 8 chromosome pairs. The density and location of signals for both 5S rDNA varied among individual chromosomes. High-intensity 5S signals were detected in the short arms of chromosomes 7 and 8, and weak signals were also detected in the long arms of the same chromosomes. For the satellite chromosomes, 17 and 18, the 5S rDNA signal was located at the end of the long arm. The third pair of chromosomes, 9 and 10, also significantly differed from other chromosomes with respect to the 5S rDNA signals observed in the subterminal region. The fourth pair of chromosomes, 11 and 12, had no 5S rDNA signal. The other pairs of chromosomes also exhibited individual signals. Our results provide information that may be beneficial for future cytogenetic studies and could contribute to the physical assembly of the Piptanthus genome.

We also recommend Trading Suppliers and Manufacturers of (5S)-3,4,5,6-Tetrahydro-5-phenyl-4(H)-1,4-oxazin-2-one (cas 144896-92-4). Pls Click Website Link as below: cas 144896-92-4 suppliers

Prev:Chromosomal and molecular characterization of 5S rRNA genes in the North American abalones Haliotis rufescens Swainson (red abalone) and H. fulgens Philippi (blue abalone)
Next:Case study concerning 5S method impact in an automotive company)