Stimulation of collagen and glycosaminoglycan production by phenytoin 5,5-diphenylhydantoin in monolayer cultures of mesenchymal cells derived from embryonic chick sternae

Add time:08/14/2019    Source:sciencedirect.com

Cultures grown with or without phenytoin (PHT) at a concentration of 5 μg/ml from the fifth to the eighth day after plating were labelled with [14C]-proline (0.2 μCi/ml) from the sixth to eighth day. Collagenase digestion indicated that collagen content increased approx. 2-fold after PHT exposure. Increases in sulphated glycosaminoglycan product in response to PHT were approx. 1.5-fold; PHT also stimulated protein production. Both actinomycin D and cycloheximide blocked incorporation of [3H]-leucine, [3H]-proline, and H235SO4 approx. 90 per cent with or without PHT. Continuous sucrose density-gradient fractionation indicated that PHT produced quantitative but not qualitative changes in cellular RNA.

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