Lanatoside 15′-O-Acetylesterase from Digitalis lanata Plants and Cell Cultures

Add time:08/19/2019    Source:sciencedirect.com

SummaryThe enzymatic deacetylation of 15′-O-acetylated cardenolides was investigated. A partially purified enzyme extract prepared from suspension-cultured Digitalis lanata cells (line K 3 OHD) showed high levels of lanatoside-hydrolysing activity. The enzyme responsible is located in the cell walls, from which it could be solubilized with 0.1 M Na-citrate buffer, pH 6.0. A standard enzyme assay was established based on an HPLC method and the apparent Km values of several cardenolide substrates were determined. Lanatoside-specific esterase could be demonstrated in leaves and several callus and suspension cultures of Digitalis lanata but not in the tissues of two other Digitalis species, namely D. purpurea and D. heywoodii.

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