Separation of dimer acids using enhanced-fluidity liquid chromatography

Add time:07/14/2019    Source:sciencedirect.com

The polymerization products of C18 fatty acids are complex mixtures of high dimeric, trimeric and higher molecular weight liquid acids. The current separation method for these mixtures involves a lengthy normal-phase HPLC gradient and the use of primary standards that are prepared via a semi-preparative HPLC. In this study, enhanced-fluidity liquid mobile phases, methanol/CO2 and methanol/CHF3, were studied as a possible alternative methodology for the separation of dimer and trimer acids. This study demonstrated that enhanced-fluidity liquid chromatography provides several advantages over normal-phase HPLC with conventional solvents. These advantages included faster speed of analysis, lower consumption of organic solvents, and much improved sample throughput.The best separation of dimer acids was achieved on a silica column using a steep methanol concentration gradient. Methanol/CO2 and methanol/CHF3 mixtures showed comparable elution strength using this steep gradient on the silica stationary phase. However, these polar compounds showed a normal-phase type retention on five reversed-phase columns which indicated an unusual retention mechanism.

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