Spectrofluorimetric determination of catecholamines with 1,2-Diphenylethylenediamine (cas 16635-95-3)

Add time:08/16/2019    Source:sciencedirect.com

A sensitive spectrolfuorimetric method for the determination of catecholamines with 1,2-diphenylethylenediamine is described. The method is based on the reaction of catechol compounds in neutral medium with 1,2-diphenylethylenediamine in the presence of hexacyanoferrate(III) and glycine at ambient temperature. The fluorescence produced shows excitation and emission maxima around 345 and 480 nm, respectively. The method is simple, selective for catechol compounds and very sensitive. Catecholamines can be determined at concentrations as low as 15–20 pmol ml−1.

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