Sequential injection determination of orthophosphate as ion associate of 12-molybdophosphate with Astra Phloxine

Add time:08/16/2019    Source:sciencedirect.com

A simple and fast reaction between 12-molybdophosphate heteropoly anion and the polymethine dye Astra Phloxine was used for the development of a new SIA method for the determination of orthophosphate. The suggested method has higher sensitivity and a broader calibration range than existing SIA methods. It can be used to detect phosphate in the range from 0.2 to 10 μmol L−1 with a detection limit of 0.1 μmol L−1 and an acceptable throughput of 20 samples h−1. The comparably low molybdate (5.6 mmol L−1) and dye (0.1 mmol L−1) concentrations led to an improvement in the stability of the base line. Inter-day reproducibility was better than 5%, while the intra-day RSD was in the range 0.8–3.5%. The method was used for the analysis of natural water samples and phosphorus-containing chemicals having a low content of orthophosphate.

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