3-Azi-1-methoxybutyl β-d-galactopyranoside, a photoaffinity label for monoclonal antigalactan antibodies of the VH gal 39.1/55.1 gene-family

Add time:08/17/2019    Source:sciencedirect.com

3-Azi-1-methoxybutyl β-d-galactopyranoside, prepared from 2,3,4,6-tetra-O-acetyl-α-d-galactopyranosyl bromide in four steps, had an affinity constant for antigalactans IgA (Fab′) J539 and X24 of 1550 and 1730 m−1, respectively. 3-Azi-1-methoxybutyl β-d-(4-3H)galactopyranoside, when photolysed in the presence of IgA X24, specifically labelled the galactan-binding area of the immunoglobulin.

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