Biosynthesis of gibberellin A12-aldehyde, gibberellin A12 and their kaurenoid precursors from [14C]mevalonic acid in a cell-free system from immature seed of Phaseolus coccineus

Add time:08/20/2019    Source:sciencedirect.com

A cell-free system capable of gibberellin (GA) biosynthesis has been prepared from immature seed of Phaseolus coccineus. This system converted [14C]mevalonic acid (MVA) to ent-kaurene, ent-kaurenoic acid, ent-Kauradienoic acid, ent-7α-hydroxykaurenoic acid, ent-6α,7α-dihydroxykaurenoic acid, GA12-aldehyde and GA12;. ent-[14C]Kaurene was converted to ent-kaurenol, ent-kaurenal, ent-kaurenoic acid and ent-7α-hydroxykaurenoic acid. All identifications were by GC/MS. The pathway from MVA to GA12-aldehyde in this species thus appears to be the same as that found in cell-free preparations derived from immature seed of other species.

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