ArticleGlobal Analysis of Intercellular Homeodomain Protein Transfer

Add time:08/18/2019    Source:sciencedirect.com

SummaryThe homeodomain is found in hundreds of transcription factors that play roles in fate determination via cell-autonomous regulation of gene expression. However, some homeodomain-containing proteins (HPs) are thought to be secreted and penetrate neighboring cells to affect the recipient cell fate. To determine whether this is a general characteristic of HPs, we carried out a large-scale validation for intercellular transfer of HPs. Our screening reveals that intercellular transfer is a general feature of HPs, but it occurs in a cell-context-sensitive manner. We also found the secretion is not solely a function of the homeodomain, but it is supported by external motifs containing hydrophobic residues. Thus, mutations of hydrophobic residues of HPs abrogate secretion and consequently interfere with HP function in recipient cells. Collectively, our study proposes that HP transfer is an intercellular communication method that couples the functions of interacting cells.

We also recommend Trading Suppliers and Manufacturers of I-POU protein (cas 138157-26-3). Pls Click Website Link as below: cas 138157-26-3 suppliers

Prev:ArticleTwin of I-POU: A two amino acid difference in the I-POU homeodomain distinguishes an activator from an inhibitor of transcription
Next:Effects of salazosulfapyridine on the profile of cell surface proteins, revealed by biotinylation of cell surface proteins and 2-dimentional electrophoresis☆)