Site-specific 15N-labelling of adenine in DNA for NMR studies
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Add time:08/20/2019 Source:sciencedirect.com
a simple and efficient protocol is described for site-specific 15N-labelling of adenine in synthetic oligodeoxynucleotides by post-synthetic substitution with 15N-labelled ammonia of the oxidized methylthio group on purine. A labelled DNA of 20-mer was characterized by NMR spectroscopy.
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