Analysis of the immune response to Yersinia enterocolitica serotype-O:9-released proteins by immunoblot and ELISA

Add time:08/25/2019    Source:sciencedirect.com

The immune response towards the released Yersinia enterocolitica outer membrane proteins (Yop) was analysed by immunoblotting and ELISA using a rabbit experimental model. Rabbits orogastrically or intravenously infected with the virulent (plasmid-bearing) Y. enterocolitica O:9 W836 strain developed a significant response by (IgG) antibodies to the released proteins having molecular weights of 51 (YopH) and 41 (LcrV) kDa, respectively. However, only in animals infected via the orogastric route were specific antibodies of the IgG class found against plasmid-encoded polypeptides of 35 (YopN) and 20 (YopQ) kDa. These results suggest that the expression of Yop in vivo may be conditioned by the route of infection used. Using ELISA, a significant response by IgG-class antibodies to YopH protein was evident in the sera from rabbits both orogastrically and intravenously infected with the virulent (pYV+) Y. enterocolitica O:9 W836 strain. By contrast, no specific antibodies to this antigen were detected in sera of rabbit infected with an avirulent (plasmid-cured) derivative (pYV−) strain. Accordingly, this protein could be very useful as an antigen in ELISA for serological diagnosis of infections caused by enteropathogenic strains of Yersinia spp.

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