Transformation of cholic acid by Arthrobacter simplex
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Add time:08/20/2019 Source:sciencedirect.com
Fermentation of cholic acid with Arthrobacter simplex (IICB 227) under aerobic conditions yielded 3,12-dioxo-23,24-dinorchola-4,6-dienoic acid, 7α-hydroxy-3,12-dioxo-23,24-dinorchol-4-enoic acid, 3α, 7α-dihydroxy-12-oxo-5β-cholan-24-oic acid, 3α,7α-dihydroxy-12-oxo-5β-23, 24-dinorcholan-22-oic acid, 7α,12α-dihydroxy-3-oxo-5β-cholan-24-oic acid, 7α-12α-dihydroxy-3-oxo-4-cholenoic acid, 7α,12α-dihydroxy-3-oxo-23, 24-dinorchol-4-enoic acid, and methyl-3α-7α, 12α-trihydroxy-5β-cholan-24-oate in addition to a new metabolite 2β-hydroxy-3,12-dioxo-23,24-dinorchola-4,6-dienoic acid. Each microbial metabolite was characterized by the application of various spectroscopic methods. The availability of some of the metabolites' enabled complete elucidation of their 13C NMR spectra.
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