Toxicomorphomics and toxicokinetics of Quinalphos (cas 13593-03-8) on embryonic development of zebrafish (Danio rerio) and its binding affinity towards hatching enzyme, ZHE1
-
Add time:08/24/2019 Source:sciencedirect.com
This study outlines the toxic effects of Quinalphos (cas 13593-03-8) (QP), an organophosphrous insecticide on the development of zebrafish (Danio rerio) embryos, with special emphasis on toxicomorphomics and toxicokinetics of target enzyme, AChE. A range of concentrations was used to elucidate the median lethal concentration (LC50) of Quinalphos. Furthermore, embryos were exposed to two sub-lethal concentrations LC10 (0.66 mg/L) and LC20 (1.12 mg/L) along with a median lethal concentration (3.0 mg/L) for 96 h. Several morphological aberrations like lordosis, kyphosis, scoliosis, heart edema, breaks in the neuronal tube and underdeveloped facial parts were noticed, which were of concentration and time dependent. The QP has adequately hindered hatching process during the course of exposure which was upheld by the in silico docking studies with hatching enzyme, ZHE1. The length of hatchlings at 96 h in LC50 concentration was significantly reduced to 47% compared to control. A significant pericardial effusion (5 to 16 fold) was observed in >90% of LC50 treated groups. Morphological changes in heart lead to the bradycardia, which ultimately leading to heart failure in some cases. The swimming behavior was significantly diminished in relation to the inhibition of AChE levels. From the in vitro kinetic studies, the kinetic constants Km, Vmax and inhibitory concentration Ki (4.45 × 10−5 M) was determined which supported the competitive nature of QP.
We also recommend Trading Suppliers and Manufacturers of Quinalphos (cas 13593-03-8). Pls Click Website Link as below: cas 13593-03-8 suppliers
Prev:Quinalphos (cas 13593-03-8) induced oxidative stress and histoarcheitectural alterations in adult male albino rats
Next:Photocatalytic degradation of Quinalphos (cas 13593-03-8) in aqueous TiO2 suspension: Reaction pathway and identification of intermediates by GC/MS) - 【Back】【Close 】【Print】【Add to favorite 】
- Related Information
- Stopped-flow and kinetic-fluorimetric determination of Quinalphos (cas 13593-03-8) in water samples08/27/2019
- Decay profile and metabolic pathways of Quinalphos (cas 13593-03-8) in water, soil and plants08/26/2019
- Photocatalytic degradation of Quinalphos (cas 13593-03-8) in aqueous TiO2 suspension: Reaction pathway and identification of intermediates by GC/MS08/25/2019
- Quinalphos (cas 13593-03-8) induced oxidative stress and histoarcheitectural alterations in adult male albino rats08/23/2019
- Development of p-nitroaniline dithiocarbamate capped gold nanoparticles-based microvolume UV–vis spectrometric method for facile and selective detection of Quinalphos (cas 13593-03-8) insecticide in environmental samples08/22/2019
- In vitro and in vivo studies on degradation of Quinalphos (cas 13593-03-8) in rats08/21/2019
- Oxidative stress response, epigenetic and behavioral alterations in Caenorhabditis elegans exposed to organophosphorus pesticide Quinalphos (cas 13593-03-8)08/20/2019
- Determination of Quinalphos (cas 13593-03-8) in human whole blood samples by high-performance thin-layer chromatography for forensic applications08/19/2019