(D-ala, D-leu) enkephalin reduces the binding of GTP in hippocampal membranes

Add time:08/22/2019    Source:sciencedirect.com

The effect of (D-Ala, D-Leu) enkephalin (DADLE) on the binding of GTP in hippocampal preparations was studied. It was observed that treatment of hippocampal slices with 10−5 −5×10−5 M DADLE followed by the preparation of membrane fractions reduced the binding of 35S-GTP-γ-S. There was no change in the affinity of the binding. This decrease of 35 S-GTP-γ-S binding was reversed when 5×10−5 M naltrindole was included. The effect was not observed when the membrane fractions were incubated with DADLE. Photoaffinity labeling with the use of 32 P P3-(4-azidoanilido) -P1 5' -GTP followed by sodium dodecyl sulfate-polycrylamide gel electrophoresis and autoradiography revealed the incorporation of radioactivity into molecular mass of the 43 kDa and 33–34 kDa proteins. 32P Photolabeling of both the 43 kDa and 33–34 kDa bands decreased following treatment of hippocampal slices with 10−4 M DADLE. These results suggested that DADLE reduces the GDP-GTP exchange in hippocampal membranes.

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