Notes & TipsFast mass spectrometry detection of tryptophan-containing peptides and proteins by reduction with pyridine–borane
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Add time:08/21/2019 Source:sciencedirect.com
In this note, we describe a method devised to detect, by means of mass spectrometry (MS), tryptophan-containing peptides and proteins using pyridine–borane. This reagent selectively reduces tryptophan residues, converting them to 2,3-dihydro-tryptophan, thereby enabling quantitation of tryptophans.
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