Gas chromatographic determination of gallopamil and norgallopamil (cas 108050-23-3) in human plasma

Add time:08/21/2019    Source:sciencedirect.com

A highly sensitive gas chromatographic assay is described for the simultaneous determination of gallopamil, a calcium channel blocking agent, and its major metabolite, norgallopamil (cas 108050-23-3). A multi-step extraction procedure is employed followed by on-column capillary gas chromatographic analysis using nitrogen-selective detection. Acetylation of norgallopamil is performed to enable accurate quantification of the metabolite. Linearity was achieved over the range 1–50 ng/ml for both analytes. Assay specificity, precision and accuracy were investigated.

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