ALL-TRANS-RETINAL (cas 116-31-4) induces Bax activation via DNA damage to mediate retinal cell apoptosis
-
Add time:08/28/2019 Source:sciencedirect.com
The current study investigates the cellular events which trigger activation of proapoptotic Bcl-2-associated × protein (Bax) in retinal cell death induced by ALL-TRANS-RETINAL (cas 116-31-4) (atRAL). Cellular events which activate Bax, such as DNA damage by oxidative stress and phosphorylation of p53, were evaluated by immunochemical and biochemical methods using ARPE-19 cells, 661 W cells, cultured neural retinas and a retinal degeneration model, Abca4−/− Rdh8−/− mice. atRAL-induced Bax activation in cultured neural retinas was examined by pharmacological and genetic methods. Other Bax-related cellular events were also evaluated by pharmacological and biochemical methods. Production of 8-OHdG, a DNA damage indicator, and the phosphorylation of p53 at Ser46 were detected prior to Bax activation in ARPE-19 cells incubated with atRAL. Light exposure to Abca4−/− Rdh8−/− mice also caused the above mentioned events in conditions of short term intense light exposure and regular room lighting conditions. Incubation with Bax inhibiting peptide and deletion of the Bax gene partially protected retinal cells from atRAL toxicity in cultured neural retina. Necrosis was demonstrated not to be the main pathway in atRAL mediated cell death. Bcl-2-interacting mediator and Bcl-2 expression levels were not altered by atRAL in vitro. atRAL-induced oxidative stress results in DNA damage leading to the activation of Bax by phosphorylated p53. This cascade is closely associated with an apoptotic cell death mechanism rather than necrosis.
We also recommend Trading Suppliers and Manufacturers of ALL-TRANS-RETINAL (cas 116-31-4). Pls Click Website Link as below: cas 116-31-4 suppliers
Prev:Research articleAll-trans retinal levels and formation of lipofuscin precursors after bleaching in rod photoreceptors from wild type and Abca4-/- mice
Next:ArticleA CEP290 C-Terminal Domain Complements the Mutant CEP290 of Rd16 Mice In Trans and Rescues Retinal Degeneration) - 【Back】【Close 】【Print】【Add to favorite 】
- Related Information
- Endocrine pharmacologyEffects of trans-resveratrol on type 1 diabetes-induced inhibition of retinoic acid metabolism pathway in retinal pigment epithelium of Dark Agouti rats08/31/2019
- Effects of quinones and flavonoids on the reduction of all-trans retinal to all-trans retinol in pig heart08/30/2019
- ArticleA CEP290 C-Terminal Domain Complements the Mutant CEP290 of Rd16 Mice In Trans and Rescues Retinal Degeneration08/29/2019
- Research articleAll-trans retinal levels and formation of lipofuscin precursors after bleaching in rod photoreceptors from wild type and Abca4-/- mice08/27/2019
- Neuroprotective effect of tetramethylpyrazine against ALL-TRANS-RETINAL (cas 116-31-4) toxicity in the differentiated Y-79 cells via upregulation of IRBP expression08/26/2019
- Research ArticleInduction of oxidative and nitrosative stresses in human retinal pigment epithelial cells by ALL-TRANS-RETINAL (cas 116-31-4)08/25/2019
- Isolation of the retinal isomers from the isomerization of ALL-TRANS-RETINAL (cas 116-31-4) by flash countercurrent chromatography☆08/24/2019
- Inhibition of interleukin-6 trans-signaling prevents inflammation and endothelial barrier disruption in retinal endothelial cells08/23/2019
- ALL-TRANS-RETINAL (cas 116-31-4) dimer formation alleviates the cytotoxicity of ALL-TRANS-RETINAL (cas 116-31-4) in human retinal pigment epithelial cells08/22/2019