Crystallization of human CCG1-interacting factor B (CIB)
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Add time:08/25/2019 Source:sciencedirect.com
A novel human protein factor CIB (C̄CG1-īnteracting factor B̄) was isolated using the yeast two-hybrid system. CIB was purified and subsequently crystallized using the vapor-diffusion method. Dynamic light-scattering studies of CIB revealed this protein to be monodispersive and present as a monomer in solution. Thin plate-like crystals were grown by the hanging-drop method in the presence of ammonium sulfate as a prime precipitant. The crystals belong to the space group P212121, with unit cell dimensions of a=43.88 Å, b=44.69 Å, and c=111.50 Å and contain one molecule in the asymmetric unit.
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