ArticleDNA Sequence Preferences of Transcriptional Activators Correlate More Strongly than Repressors with Nucleosomes

Add time:08/26/2019    Source:sciencedirect.com

SummaryTranscription factors (TFs) and histone octamers are two abundant classes of DNA binding proteins that coordinate the transcriptional program in cells. Detailed studies of individual TFs have shown that TFs bind to nucleosome-occluded DNA sequences and induce nucleosome disruption/repositioning, while recent global studies suggest this is not the only mechanism used by all TFs. We have analyzed to what extent the intrinsic DNA binding preferences of TFs and histones play a role in determining nucleosome occupancy, in addition to nonintrinsic factors such as the enzymatic activity of chromatin remodelers. The majority of TFs in budding yeast have an intrinsic sequence preference overlapping with nucleosomal histones. TFs with intrinsic DNA binding properties highly correlated with those of histones tend to be associated with gene activation and might compete with histones to bind to genomic DNA. Consistent with this, we show that activators induce more nucleosome disruption upon transcriptional activation than repressors.

We also recommend Trading Suppliers and Manufacturers of PUT3 transcriptional activator (cas 138391-78-3). Pls Click Website Link as below: cas 138391-78-3 suppliers

Prev:ArticleTranscription Factor Binding to a DNA Zip Code Controls Interchromosomal Clustering at the Nuclear Periphery
Next:Regular ArticleSynergistic Activation of Transcription by Physiologically Unrelated Transcription Factors through Cooperative DNA-Binding)