Immobilization and characterization of hemoglobin on modified sporopollenin (cas 12712-72-0) surfaces

Add time:08/29/2019    Source:sciencedirect.com

Hemoglobin was covalently immobilized onto modified sporopollenin (cas 12712-72-0) surface with different functional groups by chemical reactions to enhance binding ability of protein. In this study, the influence of various silane linker molecules on the capacity of protein binding was studied. For this purpose, activated sporopollenin was modified by 3-aminopropyltriethoxysilane (APTS), 3-chloropropyltrimethoxysilane (CPTS) and (3-glycidyloxypropyl)trimethoxysilane (GPTS). Hemoglobin (Hb) was immobilized on modified sporopollenin surfaces in phosphate buffer saline solution (PBS, pH 7.4) at 4 °C. Results showed that GPTS modified sporopollenin surfaces resulted in the highest binding capacity for Hb. Micro porosity of samples was observed through scanning electron microscopy (SEM) and thermal behavior of the samples were studied with thermogravimetric analysis (TGA) within a temperature range: 25–900 °C. TGA studies demonstrated the advantages of silane modification for high temperature applications and illustrated differences of the structures due to the different tail groups.

We also recommend Trading Suppliers and Manufacturers of sporopollenin (cas 12712-72-0). Pls Click Website Link as below: cas 12712-72-0 suppliers

Prev:Newly isolated sporopollenin (cas 12712-72-0) microcages from Platanus orientalis pollens as a vehicle for controlled drug delivery
Next:Controlled release and anti-proliferative effect of imatinib mesylate loaded sporopollenin (cas 12712-72-0) microcapsules extracted from pollens of Betula pendula)