Changes in shape and size of cat spinal root myelinated nerve fibers during fixation and Vestopal-W embedding for electron microscopy

Add time:07/14/2019    Source:sciencedirect.com

The influence of glutaraldehyde fixation, osmication, acetone dehydration, and Vestopal-W embedding on shape and dimensions of cat myelinated lumbar spinal root nerve fibers was investigated using light and electron microscopy. Glutaraldehyde fixation gave no clear changes in nerve fiber shape or dimensions. The subsequent preparative steps introduced a decrease in the whole fiber “diameter” of about 10% irrespective of the original fiber “diameter”. Myelin sheath thickness decreased by about 30–35%. With this background it was calculated that axon “diameter” should change with the g value of the fiber; viz., Y = (X − Z)/g + Z, where X, Y, and Z are terms expressing the change (%) in values for fiber “diameter”, axon “diameter”, and myelin sheath thickness, respectively. It was noted that the axon “diameter” of fibers with g values of 0.4, 0.5, 0.6, and 0.7, changed by +20, +15, +5 and 0%, respectively. Experimental data confirmed these calculations. The most striking preparatory change at the nodes of Ranvier was widening of the node gaps.

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