Original articleThe adhesive protein invasin (cas 114073-91-5) of Yersinia pseudotuberculosis induces neutrophil extracellular traps via β1 integrins

Add time:08/28/2019    Source:sciencedirect.com

Yersinia pseudotuberculosis adhesive protein invasin (cas 114073-91-5) is crucial for the bacteria to cross the intestine epithelium by binding to β1 integrins on M-cells and gaining access to the underlying tissues. After the crossing invasin can bind to β1 integrins on other cell surfaces, however effector proteins delivered by the type III secretion system Y. pseudotuberculosis efficiently inhibit potential immune responses induced by this interaction. Here, we use mutant Y. pseudotuberculosis strains lacking the type III secretion system and additionally invasin-expressing Escherichia coli to analyze neutrophil responses towards invasin. Our data reveals that invasin induces production of reactive oxygen species and release of chromatin into the extracellular milieu, which we confirmed to be neutrophil extracellular traps by immunofluorescence microscopy. This was mediated through β1 integrins and was dependent on both the production of reactive oxygen species and signaling through phosphoinositide 3-kinase. We therefore have gained insight into a potential role of integrins in inflammation and infection clearance that has not previously been described, suggesting that targeting of β1 integrins could be utilized as an adjunctive therapy against yersiniosis.

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Prev:This article is part of a Special Issue on Proteins with Tandem RepeatsThe repeat structure of two paralogous genes, Yersinia ruckeri invasin (cas 114073-91-5) (yrInv) and a “Y. ruckeri invasin (cas 114073-91-5)-like molecule”, (yrIlm) sheds light on the evolution of adhesive capacities of a fish pathogen☆
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